Induction of pluripotent stem cells of embryonic mouse and adult fibroblastic cultures with the specified factors.

Induction of pluripotent stem cells of embryonic mouse and adult fibroblastic cultures with the specified factors.

Differentiated cells can be reprogrammed to a state such as embryonic by transferring nuclear content to oocytes or with fusion with embryonic stem cells. Little is known about the factors that induce this reprogramming. Here, we show induction of pluripotent stem cells from embryo mouse or adult fibroblasts by introducing four factors, Oct3 / 4, Sox2, C-MYC, and KLF4, under the condition of the culture of ice cells. Unexpectedly, nanog can be discarded.

These cells, which we appoint IPS cells (induction of pluripotent stem), show morphology and the growth properties of ice cells and express ice marker genes. The subcutaneous transplant of IPS cells to naked mice resulted in tumors that contain various networks from the three layers of germs.

After injection becomes a blastocist, IPS cells contribute to the embryonic development of the mouse. This data shows that pluripotent stem cells can be directly generated from fibroblast culture by adding only a few factors specified.

Map and measure mammal transcriptom by RNA-SEQ.

We have mapped and measured the mouse transcriptom by sorting them and recording how often each gene is represented in the sequence sample (RNA-SEQ). This provides a digital size of the existence and prevalence of transcripts of known and previously unknown genes. We report reference measurements consisting of 41-52 million mapped 25-base-pair reap for poly (a) – RNA levels from adult mouse brains, muscle tissue and frame.

We use RNA standards to measure the prevalence of transcripts and to test the linear range of transcript detection, which stretches five magnitude. Although> 90% of the reading mapped uniquely falling in known exon, the remaining data shows a new and revised model model, including changed or additional promoter changes, exon and 3 ‘non-translated areas, and new candidate microorda precursors.

The RNA Splice event, which is not easily measured by the expression of the microarray standard gene or serial analysis of the gene expression method, is detected directly by mapping the sequence of junction connections. We observed 1.45 x 10 (5) different connections, and alternative alternative splices, with 3,500 different genes expressing one or more alternative internal connections.

Order of the human genome.

Sequence Consensus Base 2.91 billion (BP) from the Euchromatic section of the human genome is produced by the Shotgun sequencing method of all genomes. The sequence of DNA 14.8 billion BP was produced for 9 months from 27,271,853 high-quality order reading (5,11 times the genome) of the two ends of plasmid clones made from DNA from five individuals.

Two assembly strategies – assembly of all genomes and regional chromosome assembly – used, each incorporates data order from the Celera and a public-funded genome effort. Public data is embraced to the 550-BP segment to make 2.9 times coverage of the sorted genome area, without entering the bias attached to the cloning and assembly procedures used by publicly funded groups.

This brings effective coverage to assemblies up to eight times, reducing the number and size of the gap in the final assembly of what will be obtained with the coverage of 511 times. Two assembly strategies produce very similar results that mostly agree with independent mapping data. Assemblies effectively cover the Euchromatic region of human chromosomes.

More than 90% of the genome in seizure scaffolding 100,000 BP or more, and 25% of the genome in the scaffolding of 10 million BP or greater. Analysis of the order of the genome revealed 26,588 protein coding transcripts that have strong strong evidence and an additional of around 12,000 genes that are reduced computing with mouse compatibility or other weak supporting evidence.

Even though the cluster solid genes are clear, almost half the gene is dispersed in a low G + C sequence separated by a large channel from the seemingly unbodged. Only 1.1% of the genome recommended by the exon, while 24% in introns, with 75% of the genomes become intergenic DNA. Duplication of Segmental Blocks, range in size to chromosome length, abundant throughout the genome and reveal the history of complex evolution.

Induction of pluripotent stem cells of embryonic mouse and adult fibroblastic cultures with the specified factors.

The comparative genome analysis shows genital vertebrata expansion associated with neuron functions, with network-specific development regulations, and with hemostasis and immune systems. The comparison of the DNA sequence between the order of consensus and the public funded genome data provides a location of 2.1 million single nucleotide polymorphism (SNP).

GAPDH Antibody - HRP

T0004-HRP 1ml
EUR 920
Description: GAPDH Mouse Monoclonal antibody detects endogenous levels of total GAPDH protein.

Giemsa stain

20-abx082069
  • EUR 175.00
  • EUR 217.00
  • 1 g
  • 5 g
  • Shipped within 5-10 working days.

Giemsa stain

20-abx082518
  • EUR 175.00
  • EUR 217.00
  • 1 g
  • 5 g
  • Shipped within 5-10 working days.

Jenner's Stain

GT5545-100G 100 g
EUR 160

Jenner's Stain

GT5545-25G 25 g
EUR 81

Giemsa Stain

GT6821-100ML 100 ml
EUR 46

Giemsa Stain

GT6821-250ML 250 ml
EUR 59

Wright's stain

GT7819-100G 100 g
EUR 110

Wright's stain

GT7819-10G 10 g
EUR 46

Wright's stain

GT7819-250G 250 g
EUR 190

Wright's stain

GT7819-25G 25 g
EUR 58

DiI Stain

B2742-250 250 mg
EUR 753

DiI Stain

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EUR 227

Giemsa stain

GB0477 10g
EUR 60.44
  • Product category: Biochemicals/Indicators/Stains/Biological Stains

CRF Anti-Polyvalent HRP Polymer (DAB) Stain Kit

CPH080 80 Slides
EUR 156

HRP-IgG Conjugation Kit

55R-HRP 1 mg
EUR 705
Description: HRP conjugation kit for all types of IgG

beta-Actin Antibody - HRP

T0022-HRP 1ml
EUR 920
Description: beta-Actin Mouse Monoclonal antibody detects endogenous levels of total beta-Actin protein.

Goat Anti-Rat IgG1-HRP Conjugate

50121-HRP 0.5 ml
EUR 225

Goat Anti-Rat IgG2a-HRP Conjugate

50122-HRP 0.5 ml
EUR 225

Goat Anti-Rat IgG2b-HRP Conjugate

50123-HRP 0.5 ml
EUR 225

Goat Anti-Rat IgG2c-HRP Conjugate

50124-HRP 0.5 ml
EUR 225

Monoclonal Anti-Rat IgG1-HRP Conjugate

50126-HRP 0.5 ml
EUR 286

Rabbit Anti-Bird IgM-HRP Conjugate

90521-HRP 100 ul
EUR 347

Concanavalin A Lectin (ConA)-HRP conjugate

CONA15-HRP 0.5 ml
EUR 286

Aleuria Aurantia Lectin (Aal)-HRP conjugate

AAL15-HRP 0.5 ml
EUR 225

Monoclonal Anti-Poly His-HRP conjugate

HISP12-HRP 100 ul
EUR 347

Lens culinaris Lectin (LCA)-HRP conjugate

LCA15-HRP 0.5 ml
EUR 286

Anti-Per.C6Ð’ Affinity Purified HRP, Goat

PC118AF-HRP 1 mg
EUR 4069
  • Product category: Antibodies
Description: Anti-Per.C6Ð’ Affinity Purified HRP, Goat by Cygnus Technologies is available in Europe via Gentaur.

Rabbit Anti-Salmonella IgG-HRP conjugate

SLM11-HRP 0.5 ml
EUR 347

Sambucus nigra Lectin (SNA)-HRP conjugate

SNA15-HRP 0.5 ml
EUR 286

Protein Stain-EZ C, Reversible Copper Stain Kit

BSP017 1kit, 10prep
EUR 74.36
  • Product category: Biochemicals/Indicators/Stains/Peptide/Protein Related

Protein Stain-EZ B, Reversible Zinc Stain Kit

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  • Product category: Molecular Biology Kits/Protein - Staining

Protein Stain-EZG Rapid Coomassie Blue Stain Solution

BSP041 1KIT, 10prep
EUR 76.1
  • Product category: Molecular Biology Kits/Protein - Staining

Silver Stain kit

AR0171 1 kit (for 30 assays to stain the gel of 5 X8.5)
EUR 152

Copper Stain Kit

CSK-1 1 kit(s)
EUR 189

Copper Stain Kit

CSK-2 1 kit(s)
EUR 120

ClearSight DNA Stain

BH40501 1ml
EUR 103
Description: For DNA staining and vizualization.

ClearSight RNA Stain

BH40601 400µl
EUR 77
Description: For DNA staining and vizualization.

Giemsa stain, powder

GT7801-100G 100 g
EUR 134

Giemsa stain, powder

GT7801-10G 10 g
EUR 46

Giemsa stain, powder

GT7801-25G 25 g
EUR 62

Giemsa stain, powder

GT7801-50G 50 g
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Giemsa stain, powder

GT7801-5G 5 g
EUR 42

Reticulum Stain Kit

GRT-1 1 kit(s)
EUR 255

Reticulum Stain Kit

GRT-2 1 kit(s)
EUR 152

Gram Stain Kit

GSK-1 125 ml ea.
EUR 125

Gram Stain Kit

GSK-2 30 ml ea.
EUR 92

Gram Stain Kit

GSK-500 500 ml ea.
EUR 303

Jenner's Stain, certified

GT1148-25G 25 g
EUR 107

Fite's Stain Kit

FLS-1 125 ml ea.
EUR 149

Fite's Stain Kit

FLS-500 500 ml ea.
EUR 295

GMS Stain Kit

KAA-1 125 ml ea.
EUR 216

GMS Stain Kit

KAA-1000 1000 ml ea.
EUR 884

GMS Stain Kit

KAA-500 500 ml ea.
EUR 523

Iron Stain Kit

IRN-1 1 kit(s)
EUR 149

Iron Stain Kit

IRN-2 100 Slides
EUR 101

Fite's Stain Kit

K1425-125
EUR 370

Geimsa Stain Kit

K1426-30
EUR 289

Geimsa Stain Kit

K1426-500
EUR 468

GMS Stain Kit

K1427-125
EUR 446

Gram Stain Kit

k1428-125
EUR 392

Gram Stain Kit

k1428-30
EUR 262

Orcein Stain Kit

K1432-125
EUR 501

Orcein Stain Kit

K1432-30
EUR 359

Pneumocystis Stain Kit

K1434-125
EUR 425

Pneumocystis Stain Kit

K1434-30
EUR 316

Steiner Stain Kit

K1435-125
EUR 446

Papanicolaou Stain Kit

K1440-30
EUR 207

Papanicolaou Stain Kit

K1440-500
EUR 278

Pneumocystis Stain Kit

PCS-1 1 kit(s)
EUR 156

Pneumocystis Stain Kit

PCS-2 100 Slides
EUR 104

Trichrome Stain (Blue)

TGB125 125 ml
EUR 74

Trichrome Stain (Blue)

TGB500 500 ml
EUR 116

Trichrome Stain (Blue)

TGB999 1000 ml
EUR 143

4CN Stain Kit

PW024 5Preps, 5prep
EUR 70.88
  • Product category: Molecular Biology Kits/Protein - Staining

TMB Stain Kit

PW025 5Preps, 5prep
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  • Product category: Molecular Biology Kits/Protein - Staining

Goat Anti-Donkey IgG (Fc)-HRP conjugate

80950-HRP 0.5 ml
EUR 225

Rabbit Anti-bovine Catalase IgG-HRP Conjugate

CATB11-HRP 100 ul
EUR 469

Sheep Anti-rat albumin IgG-HRP Conjugate

ALBR12-HRP 100 ul
EUR 347

Goat Anti-human lactoferrin IgG-HRP Conjugate

LTF11-HRP 0.5 ml
EUR 445

Goat Anti-bovine lactoferrin IgG-HRP conjugate

LTF13-HRP 0.5 ml
EUR 445

Chicken Anti-Protein-A IgG-HRP conjugate

PRTA13-HRP 100 ul
EUR 347

Recombinant (E.Coli) purified Protein G-HRP Conjugate

PRTG15-HRP 1 ml
EUR 225

Monoclonal Anti-Phosphotyrosine (PY20) IgG-HRP conjugate

PTYR12-HRP 100 ul
EUR 408

Goat Anti-Human Transferrin IgG-HRP conjugate

TF11-HRP 100 ug
EUR 347

Goat Anti-Mouse Transferrin IgG-HRP conjugate

TF14-HRP 100 ug
EUR 347

Wheat germ agglutinin Lectin (WGA)-HRP conjugate

WGA15-HRP 0.5 ml
EUR 286

Goat Anti-Turkey IgG (H+L)-HRP conjugate

60600-HRP 0.5 ml
EUR 225

Goat Anti-Mouse IgG (Fc) IgG-HRP Conjugate

40151-HRP 1 ml
EUR 225

Goat Anti-Diphtheria Toxoid/Toxin IgG-HRP conjugate

DTOX12-HRP 0.5 ml
EUR 408

Rabbit Anti-Donkey IgG (H+L)-HRP conjugate

80920-HRP 1 ml
EUR 213

Rabbit Anti-bovine Adenosine deaminase IgG-HRP conjugate

ADA11-HRP 100 ul
EUR 384

Goat Anti-Human Plasma Fibrinogen IgG-HRP Conjugate

FIBN17-HRP 100 ul
EUR 408

Goat Anti-Luciferase (Fire fly) IgG-HRP Conjugate

LUCF11-HRP 100 ul
EUR 347

Rabbit Anti-Luciferase (Photobacterium fischerii) IgG-HRP Conjugate

LUCF12-HRP 500 ul
EUR 384

Monoclonal Anti-Maltose binding protein (MBP)-HRP conjugate

MBP11-HRP 100 ul
EUR 347

Goat Anti-Human plasminogen protein IgG, HRP conjugate

PLMN12-HRP 100 ul
EUR 408

Ricinus communis (castor bean) Agglutinin (RCA120)-HRP conjugate

RCA15-HRP 0.5 ml
EUR 286

Rabbit Anti-Human alpha-Tubulin IgG-HRP Conjugate

TUBA13-HRP 100 ul
EUR 432

Goat Anti-Adenovirus type 2, hexon IgG-HRP conjugate

ADV11-HRP 0.5 ml
EUR 408

Rabbit anti-Beta-Galactosidase (E. Coli) IgG-HRP conjugate

BGAL12-HRP 100 ul
EUR 347

Goat Anti-Hepatitis Surface Antigen (HBsAg) IgG-HRP Conjugate

HBA11-HRP 1 ml
EUR 347

Mouse Monoclonal Anti-Hepatitis Surface Antigen (HBsAg)-HRP Conjugate

HBA12-HRP 1 ml
EUR 347

Rabbit Anti-human spleen ferrtin protein IgG, HRP conjugate

FERT11-HRP 100 ul
EUR 408

Goat Anti-Poliomyelitis Virus 1-3 IgG-HRP Conjugate

POLV13-HRP 0.5 ml
EUR 408

Goat Anti-Rubella virus (HPV77 strain) IgG-HRP conjugate

RUBL11-HRP 1 ml
EUR 347

Protein Stain-EZ A Ultra Sensitive Coomassie Blue Stain Solution

SK3011 10Minigel, 10UNIT
EUR 66.53
  • Product category: Biochemicals/Indicators/Stains/Peptide/Protein Related

Rabbit Anti-Human beta 2-microglobulin protein IgG, HRP conjugate

B2M11-HRP 100 ul
EUR 408

Rabbit Anti-Bovine Serum Albumin (BSA) protein IgG-HRP Conjugate

ALBB14-HRP 0.5 ml
EUR 347

Goat Anti-Human Alpha 1-Antitrypsin (A1AT) IgG, HRP conjugate

A1AT11-HRP 100 ul
EUR 408

Goat Anti-Hemeagglutinin fusion epitope (HA-tag) IgG-HRP conjugate

HA12-HRP 100 ug
EUR 347

Goat Anti-Glutathione Transferase (GST, S. japonicum) IgG-HRP Conjugate

GST13-HRP 100 ul
EUR 347

Bovine Fetuin (Alpha-2 HS-Glycoprotein, AHSG, A2HS)-HRP Conjugate

FETB15-HRP 500 ul
EUR 286

Rabbit Anti-c-Myc epitope (fusion tag) IgG-HRP conjugate

MYC13-HRP 100 ul
EUR 347

Goat Anti-Human Respiratory Syncytial Virus (RSV) IgG-HRP conjugate

RSV12-HRP 0.5 ml
EUR 469

Monoclonal Anti-Vesicular Stomatitis Virus Glycoprotein (VSV)-IgG-HRP conjugate

VSV11-HRP 100 ul
EUR 347

EA-50 Stain Solution

EAC3800 1 Gal.
EUR 183

EA-50 Stain Solution

EAC500 500 ml
EUR 88

EA-50 Stain Solution

EAC999 1000 ml
EUR 111

EA-65 Stain Solution

EAD3800 1 Gal.
EUR 154

EA-65 Stain Solution

EAD500 500 ml
EUR 84

EA-65 Stain Solution

EAD999 1000 ml
EUR 101

Colloidal Iron Stain Kit

CIK-1 1 kit(s)
EUR 156

Colloidal Iron Stain Kit

CIK-2 100 Slides
EUR 109

Bielshowsky's Stain Kit (Modified)

BSK-1 1 kit(s)
EUR 513

InstaBlue Protein Stain Solution

B8226-1000 1 L
EUR 108
Description: InstaBlue Protein Stain Solution is a ready to use Coomassie protein stain for polyacrylamide gels. Its unique patented mechanism of action stains proteins in 5 minutes while leaving a clear background without the need to fix, wash or destain.

InstaBlue Protein Stain Solution

B8226-500 500 ml
EUR 84
Description: InstaBlue Protein Stain Solution is a ready to use Coomassie protein stain for polyacrylamide gels. Its unique patented mechanism of action stains proteins in 5 minutes while leaving a clear background without the need to fix, wash or destain.

Giemsa stain, powder, certified

GT5290-100G 100 g
EUR 178

Giemsa stain, powder, certified

GT5290-10G 10 g
EUR 62

Giemsa stain, powder, certified

GT5290-25G 25 g
EUR 86

Giemsa stain, powder, certified

GT5290-50G 50 g
EUR 118

Giemsa stain, powder, certified

GT5290-5G 5 g
EUR 50

EvaGreenFluorescent DNA Stain (100µM)

F253 1 ml
EUR 121

EvaGreenFluorescent DNA Stain (100µM)

F253L 5x1 ml
EUR 484

Modified Gram Stain Kit

K1429-125
EUR 338

Modified Gram Stain Kit

K1429-30
EUR 251

Random pairs of human haploid genomes are different at the level 1 of BP per 1250 on average, but there are heterogeneity marked at the level of polymorphism throughout the genome. Less than 1% of all SNP results in variations in protein, but the task determines which SNP which has functional consequences remains an open challenge.

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