Corticosteroids, ionoffor calcium and anti-CD3 monoclonal antibodies kill simoocyte mice incubated in vitro. Cell death is preceded by extensive DNA fragmentation into the oligonucleosomom subunit. This type of death (apoptosis), which is physiologically occurring in the intratrate process of immune cell selection, is usually evaluated by electrophoresis or colorimetric methods that measure DNA fragmentation in nuclear extracts. These techniques cannot determine the percentage of apoptotic nucleetics or recognize apoptotic cells in the population of heterogeneous cells.
We have developed a cytometric flow method to measure apoptotic nucleetic percentages after coloring propidium iodide in hypotonic buffers and has compiled it with classical columnic techniques and electrofores using Dexamethasone (DEX). ApopTotic Nuclei emerged as the culmination of extensive hypodiploid DNA which was easily discriminated against from the narrow peak of thymocytes with a normal (diploid) DNA content in the red fluorescence channel. When Dex induced apoptosis is inhibited by low temperature incubation (4 degrees C) or cycloheximide treatment, no peak of the hypodiploid DNA appears.
Likewise, thymocyte death caused by sodium azide, a substance with cell murder activities through non-apoptotic mechanisms, does not produce any variations in the peak of normal DNA. Sitometric flow data shows a very good correlation with the results obtained by electrophoretic and colorimetric methods. This new, simple and simple method must prove useful to assess certain population apoptosis in heterogeneous tissue such as bone marrow, thymus and lymph nodes.
Macrophage alternative activation: Immunological functional perspective.
Macrophages are default immune cells with established roles in the main response to pathogens, but also in network homeostasis, coordination of adaptive immune response, inflammation, resolution, and repairs. These cells recognize danger signals through receptors that are able to induce special activation programs. Activation of macrophages known to be induced classically by IFN-Gamma, which triggered a hard proinflammatory response needed to kill intracellular pathogens.
Macrophages also undergo alternative activation by IL-4 and IL-13, which trigger different phenotypes that are important for immune responses to parasites. Here we review this cytokine cellular source, receptor signal path, and mark markers and genutors of genes. We draw attention to differences found between the mouse and alternative human activation model. Evidence for in vivo alternative macrophages activation was also analyzed, with nematode infection as a prototypic disease. Finally, we revisit the concept of macrophage activation in the context of the immune response.
In the analysis of Vivo Autophagy in response to nutritional hunger using transgenic mice that expressed the automophagosome fluorescent marker.
Macroautophagy mediates mass degradation of cytoplasmic components. It accounts for degradation of the longest protein aged: cytoplasmic constituents, including organelles, exiled to autofagosomes, which are then fuses with lisosomes, where relegation occurs. Although the possibility of autophagy involvement in homeostasis, development, cell death, and pathogenesis has been repeatedly shown, systematically in Vivo analysis has not been carried out in mammals, especially due to limited monitoring methods.
To understand where and when autophagy occurs in Vivo, we have produced transgenic mice that express GFP systemically integrated with LC3, which is a mammalian homologous from ATG8 yeast (AUT7 / APG8) and serves as a marker protein for autophagosomes. Fluorescence microscopic analysis reveals that different autophagies are induced by nutrean hunger in most tissues.
On several networks, Autophagy even occurs actively without starvation. Our results show that autophagy regulations depend on organs and the role of autophagy are not limited to hunger response. This transgenic mouse model is a useful tool for studying autophagy mammals.
The generation of functional milk glands of single stem cells.
The existence of mammary stem cells (Mascs) has been postandaken from evidence that the mammary gland can be regenerated by transplantating epithelial fragments in mice. Interest in Mascs has been further distimulated with their potential role in breast tumorigenesis. However, the identity and purification of Mascs has proven difficult to understand because of the lack of specified markers.
We isolate the discrete population of mammarus cells based on cell surface markers and identify subpopulations (LIN-CD29HICD24 +) which are highly enriched for mascs with transplantation. Here we show that one cell, is marked with Transgene Lacz, can reorganize the complete mammary gland in Vivo.
Transplant cells contribute to the lines of luminal and myoepithelial and generated functional lobuloalveolar units during pregnancy. The capacity of the destruction of these cells is indicated by serial transplants from clone results. In supporting the potential role for Mascs in breast cancer, subpared subpopulation by the parent is expanded in premalignant milk tissue from MMTV-WNT-1 mice and contains a higher number of mascs.
 EA-65 Stain Solution |
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| EAD500 | ScyTek Laboratories | 500 ml | EUR 84 |
EA-65 Stain Solution |
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| EAD999 | ScyTek Laboratories | 1000 ml | EUR 101 |
) Trichrome Stain Solution (Green) |
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| TGG125 | ScyTek Laboratories | 125 ml | EUR 88 |
Trichrome Stain Solution (Green) |
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| TGG500 | ScyTek Laboratories | 500 ml | EUR 116 |
Trichrome Stain Solution (Green) |
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| TGG999 | ScyTek Laboratories | 1000 ml | EUR 143 |
 STAT-Q Mouse Adsorbed AEC KIT; for staining Rat antibodies-on-Mouse tissues 250 plus slide kit |
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| NB314MAK-AEC | Innovex | 200-250 slide kit | EUR 856 |
 STAT-Q Rat Adsorbed AEC Kit: for staining Mouse antibodies-on-Rat tissues 250 plus slides kit |
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| NB314RAK-AEC | Innovex | 200-250 slide kit | EUR 856 |
 Protein Stain-EZG Rapid Coomassie Blue Stain Solution |
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| BSP041 | Bio Basic | 1KIT, 10prep | EUR 76.1 |
) Differentiating Solution (For Orcein Stain) |
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| DSA125 | ScyTek Laboratories | 125 ml | EUR 71 |
Differentiating Solution (For Orcein Stain) |
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| DSA500 | ScyTek Laboratories | 500 ml | EUR 122 |
 Methylene blue Solution, 100x DNA stain |
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| ME622 | Bio Basic | 25ml | EUR 93.5 |
) Oxidizer Solution (For Steiner's Silver Stain) |
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| SOS125 | ScyTek Laboratories | 125 ml | EUR 76 |
Oxidizer Solution (For Steiner's Silver Stain) |
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| SOS500 | ScyTek Laboratories | 500 ml | EUR 111 |
 T-Pro EZ stain Gel solution |
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| JT90-R005M | T-Pro Biotechnology | 500ml/BT | EUR 187 |
T-Pro EZ stain Gel solution |
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| JT90-R005S | T-Pro Biotechnology | 100ml/BT | EUR 126 |
 Protein Stain-EZ A Ultra Sensitive Coomassie Blue Stain Solution |
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| SK3011 | Bio Basic | 10Minigel, 10UNIT | EUR 66.53 |
 AEC |
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| 2122-10 | Biovision | EUR 164 | |
 pUC57- Simple |
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| PVT10561 | Lifescience Market | 2 ug | EUR 266 |
 pUC57 Simple Plasmid |
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| PVT0006 | Lifescience Market | 2 ug | EUR 325 |
) pMD- 19T (Simple) |
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| PVT0016 | Lifescience Market | 2 ug | EUR 266 |
 T1 Simple Cloning Kit |
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| 20-abx098056 | Abbexa |
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 Blunt Simple Cloning Kit |
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| 20-abx098060 | Abbexa |
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 pUC57 Simple-PVT1 Plasmid |
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| PVTB00511 | Lifescience Market | 2 ug | EUR 356 |
 pUC57 Simple-Pdcd4 Plasmid |
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| PVTB70003 | Lifescience Market | 2 ug | EUR 356 |
 pUC57- Simple- gRNA backbone |
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| PVT11371 | Lifescience Market | 2 ug | EUR 301 |
 AEC Substrate Kit |
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| AR1020 | BosterBio | 3mL X 2 (for 600-900 assays) | EUR 71 |
 AEC Chromogen Kit |
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| abx097196-1Kit | Abbexa | 1 Kit | EUR 272 |
 AEC Chromogen Concentrate |
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| A3610-002 | GenDepot | 20ml | EUR 178 |
AEC Chromogen Concentrate |
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| A3610-005 | GenDepot | 50ml | EUR 286 |
AEC Chromogen Concentrate |
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| A3610-010 | GenDepot | 100ml | EUR 448 |
 AEC coloring system |
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| CT356 | U-CyTech | 10-plate | EUR 141 |
 AEC Enhancer, 15ml |
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| NB319 | Innovex | 15 ml | EUR 222 |
AEC Chromogen Concentrate |
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| ACD015 | ScyTek Laboratories | 15 ml | EUR 102 |
AEC Chromogen Concentrate |
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| ACD030 | ScyTek Laboratories | 30 ml | EUR 148 |
AEC Chromogen Concentrate |
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| ACD125 | ScyTek Laboratories | 125 ml | EUR 245 |
AEC Chromogen Concentrate |
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| ACD500 | ScyTek Laboratories | 500 ml | EUR 671 |
 AEC Substrate Buffer |
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| ACE010 | ScyTek Laboratories | 10 L | EUR 316 |
AEC Substrate Buffer |
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| ACE500 | ScyTek Laboratories | 500 ml | EUR 72 |
AEC Substrate Buffer |
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| ACE999 | ScyTek Laboratories | 1000 ml | EUR 80 |
AEC Substrate Buffer |
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| 2123-50 | Biovision | EUR 137 | |
 2-component AEC system, 70ml) AEC Chromogen & Substrate (stable) 2-component AEC system, 70ml |
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| NB314SCS | Innovex | 70 ml | EUR 450 |
 Cyber Orange™ Nucleic Acid Gel Stain *10,000X DMSO Solution* |
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| 17595 | AAT Bioquest | 1 ml | EUR 306 |
 Gelite™ Red Nucleic Acid Gel Stain *10,000X DMSO Solution* |
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| 17600 | AAT Bioquest | 1 mL | EUR 176 |
 Gelite™ Red Nucleic Acid Gel Stain *100X Water Solution* |
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| 17603 | AAT Bioquest | 10 mL | EUR 132 |
 AEC Chromogen/Substrate Kit |
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| A3611-500 | GenDepot | 500 slides | EUR 188 |
AEC Chromogen/Substrate Kit |
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| ACG500 | ScyTek Laboratories | 500 Slides | EUR 85 |
 AEC Chromogen/Substrate Bulk Kit |
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| ACJ500 | ScyTek Laboratories | 515 ml | EUR 120 |
AEC Chromogen/Substrate Bulk Kit |
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| ACJ999 | ScyTek Laboratories | 1030 ml | EUR 176 |
 Giemsa stain |
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| GB0477 | Bio Basic | 10g | EUR 60.44 |
Giemsa stain |
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| 20-abx082069 | Abbexa |
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Giemsa stain |
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| 20-abx082518 | Abbexa |
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 Jenner's Stain |
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| GT5545-100G | Glentham Life Sciences | 100 g | EUR 160 |
Jenner's Stain |
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| GT5545-25G | Glentham Life Sciences | 25 g | EUR 81 |
 Giemsa Stain |
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| GT6821-100ML | Glentham Life Sciences | 100 ml | EUR 46 |
Giemsa Stain |
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| GT6821-250ML | Glentham Life Sciences | 250 ml | EUR 59 |
 Wright's stain |
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| GT7819-100G | Glentham Life Sciences | 100 g | EUR 110 |
Wright's stain |
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| GT7819-10G | Glentham Life Sciences | 10 g | EUR 46 |
Wright's stain |
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| GT7819-250G | Glentham Life Sciences | 250 g | EUR 190 |
Wright's stain |
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| GT7819-25G | Glentham Life Sciences | 25 g | EUR 58 |
 DiI Stain |
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| B2742-250 | Biovision | 250 mg | EUR 753 |
DiI Stain |
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| B2742-50 | Biovision | 50 mg | EUR 227 |
 Protein Stain-EZ C, Reversible Copper Stain Kit |
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| BSP017 | Bio Basic | 1kit, 10prep | EUR 74.36 |
 Protein Stain-EZ B, Reversible Zinc Stain Kit |
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| BSP019 | Bio Basic | 1kit, 10prep | EUR 74.36 |
 Silver Stain kit |
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| AR0171 | BosterBio | 1 kit (for 30 assays to stain the gel of 5 X8.5) | EUR 152 |
 4CN Stain Kit |
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| PW024 | Bio Basic | 5Preps, 5prep | EUR 70.88 |
 TMB Stain Kit |
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| PW025 | Bio Basic | 5Preps, 5prep | EUR 70.88 |
 ClearSight DNA Stain |
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| BH40501 | Bioatlas | 1ml | EUR 103 |
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Description: For DNA staining and vizualization. |
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 ClearSight RNA Stain |
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| BH40601 | Bioatlas | 400µl | EUR 77 |
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Description: For DNA staining and vizualization. |
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 Copper Stain Kit |
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| CSK-1 | ScyTek Laboratories | 1 kit(s) | EUR 189 |
Copper Stain Kit |
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| CSK-2 | ScyTek Laboratories | 1 kit(s) | EUR 120 |
 Jenner's Stain, certified |
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| GT1148-25G | Glentham Life Sciences | 25 g | EUR 107 |
 Giemsa stain, powder |
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| GT7801-100G | Glentham Life Sciences | 100 g | EUR 134 |
Giemsa stain, powder |
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| GT7801-10G | Glentham Life Sciences | 10 g | EUR 46 |
Giemsa stain, powder |
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| GT7801-25G | Glentham Life Sciences | 25 g | EUR 62 |
Giemsa stain, powder |
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| GT7801-50G | Glentham Life Sciences | 50 g | EUR 86 |
Giemsa stain, powder |
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| GT7801-5G | Glentham Life Sciences | 5 g | EUR 42 |
 Pneumocystis Stain Kit |
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| PCS-1 | ScyTek Laboratories | 1 kit(s) | EUR 156 |
Pneumocystis Stain Kit |
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| PCS-2 | ScyTek Laboratories | 100 Slides | EUR 104 |
) Trichrome Stain (Blue) |
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| TGB125 | ScyTek Laboratories | 125 ml | EUR 74 |
Trichrome Stain (Blue) |
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| TGB500 | ScyTek Laboratories | 500 ml | EUR 116 |
Trichrome Stain (Blue) |
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| TGB999 | ScyTek Laboratories | 1000 ml | EUR 143 |
 Fite's Stain Kit |
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| FLS-1 | ScyTek Laboratories | 125 ml ea. | EUR 149 |
Fite's Stain Kit |
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| FLS-500 | ScyTek Laboratories | 500 ml ea. | EUR 295 |
 Iron Stain Kit |
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| IRN-1 | ScyTek Laboratories | 1 kit(s) | EUR 149 |
Iron Stain Kit |
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| IRN-2 | ScyTek Laboratories | 100 Slides | EUR 101 |
 GMS Stain Kit |
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| KAA-1 | ScyTek Laboratories | 125 ml ea. | EUR 216 |
GMS Stain Kit |
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| KAA-1000 | ScyTek Laboratories | 1000 ml ea. | EUR 884 |
GMS Stain Kit |
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| KAA-500 | ScyTek Laboratories | 500 ml ea. | EUR 523 |
 Reticulum Stain Kit |
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| GRT-1 | ScyTek Laboratories | 1 kit(s) | EUR 255 |
Reticulum Stain Kit |
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| GRT-2 | ScyTek Laboratories | 1 kit(s) | EUR 152 |
 Gram Stain Kit |
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| GSK-1 | ScyTek Laboratories | 125 ml ea. | EUR 125 |
Gram Stain Kit |
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| GSK-2 | ScyTek Laboratories | 30 ml ea. | EUR 92 |
Our data specifies that single cells in the LIN-CD29HICD24 + population are multipotent and own updates, the properties that define them as Mascs.